Coding
Part:BBa_K5477022:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
POR - Cytochrome P450 oxidoreductase codon optimized for Saccharomyces cerevisiae
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377
Illegal NgoMIV site found at 699
Illegal NgoMIV site found at 818 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our system, POR plays a critical role in enabling the CYP3A4 and other cytochrome P450 enzymes to function effectively.
Source
Homo sapiens