Part:BBa_K5477009

Lex6Op - six tandem copies of LexA operator

The Lex6Op refers to six tandem copies of the LexA operator. Transcription factors that has a LexA DNA-binding domain can bind the operator sequences and induce transcriptional activation of the promoter upon presence of the ligand (1) (2).

This part was used in the reporter module BBa_K5477031 where the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with the wildtype or mutant ERα will bind to these operators that drive the expression of the reporter gene NanoLuc. The devices that contain this part are: BBa_K5477043, BBa_K5477044, BBa_K5477045 and BBa_K5477046.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Shaw, W.M., et al., Engineering a Model Cell for Rational Tuning of GPCR Signaling. Cell, 2019. 177(3): p. 782-796 e27.

2. Zhou, T., Liang, Z. & Marchisio, M.A. Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol. Sci Rep 12, 21791 (2022). https://doi.org/10.1038/s41598-022-26195-x