Part:BBa_K5477001
pSTE12 - constitutive promoter in Saccharomyces cerevisiae
STE12 is a transcription factor activated by the MAPK signaling cascade, responsible for inducing genes involved in mating and pseudohyphal/invasive growth pathways in Saccharomyces cerevisiae. It works in conjunction with the transcription factor Tec1p to regulate genes specific to invasive growth (1) (2). Although typically regulated by environmental signals. pSTE12 promoter was utilized as a constitutive promoter, driving expression of receptor modules independent of external signaling. Instead, pSTE12 was used in the receptor module for AhR | BBa_K5477023.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 55
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 55
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 55
- 1000COMPATIBLE WITH RFC[1000]
References
1. Bardwell L, Cook JG, Voora D, Baggott DM, Martinez AR, Thorner J. Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev. 1998 Sep 15;12(18):2887-98. doi: 10.1101/gad.12.18.2887. PMID: 9744865; PMCID: PMC317171.
2. Roberts RL, Fink GR. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 1994 Dec 15;8(24):2974-85. doi: 10.1101/gad.8.24.2974. PMID: 8001818.
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