Terminator

Part:BBa_K5471008:Design

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)

_NOTOC__ SV40 polyA singal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 57
    Illegal NotI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was excised from an already commercially available vector, and was then immediately ready for use.


Source

This part is directly taken from already commercially available vector pSilencer 4.1-CMV neo.

References

Ghosh T, Soni K, Scaria V, Halimani M, Bhattacharjee C, Pillai B. MicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic {beta}-actin gene. Nucleic Acids Res. 2008 Nov;36(19):6318-32. doi: 10.1093/nar/gkn624. Epub 2008 Oct 3. PMID: 18835850; PMCID: PMC2577349.