Coding

Part:BBa_K5471007

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


eGFP

This part is eGFp gene's coding region, encoding the protein enhanced GFP (eGFP), a green fluorescent protein originally derived from the jellyfish Aequorea victoria, and modified to produce a brighter signal when hit with blue or ultraviolet wavelengths of light

This part can be used as a reporter gene, providing fluorescence as a means to verify successful genetic engineering.

This part's sequence was used for the construction of the pAAV-EF1a-mir195-eGFP-SV40pA plasmid https://parts.igem.org/Part:BBa_K5471020 our team used for our experiments

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None