Part:BBa_K5466033

Triple fluorescence tricistronic device

A device that constitutively produces three fluorescent proteins—mCherry, spGFP, and mCerulean3—under a single promoter to test the behavior of IGG6/NAL10.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

IGG6/Nal10

To reduce the length and complexity of genetic constructs, systems for polycistronic expression are required, permitting the expression of several genes from a single promoter and avoiding a multiple promoter strategy.

Thanks to the iGEM Fluorescence Measurement Calibrants Kit, it is possible to convert OD600 and fluorescence intensity units into absolute units. This would allow for the comparison of the effect of position in a polycistronic gene, thanks to IGG6, on translation.

References

Ma, X., Yue, Q., Miao, L., Li, S., Tian, J., Si, W., Zhang, L., Yang, W., Zhou, X., Zhang, J., Chen, R., Xu, Y., & Liu, X. (2024). A novel nucleic acid linker for multi‐gene expression enhances plant and animal synthetic biology. The Plant Journal, 118(6), 1864–1871. https://doi.org/10.1111/tpj.16714

Yue, Q., Meng, J., Qiu, Y., Yin, M., Zhang, L., Zhou, W., An, Z., Liu, Z., Yuan, Q., Sun, W., Li, C., Zhao, H., Molnár, I., Xu, Y., & Shi, S. (2023). A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi. Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-40027-0