Device

Part:BBa_K5466028

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-24)


RFP expression controlled by CadC

Expression of RFP (BBa_B1010) induced upon dimerization of CadC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 720
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Reporter

We use a reporter RFP to visually indicate the presence of aflatoxin in the environment. When AFB1 is detected, the expression of RFP provides a clear signal, allowing us to monitor the response in real time. This serves as both a confirmation that the detection system is actively responding to the toxin and a convenient method to track the process without the need for complex or time-consuming assays. The fluorescence of RFP acts as a reliable indicator, ensuring that the system is functioning as intended in various environments


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None