Composite

Part:BBa_K5444009

Designed by: Ihsiu Huang   Group: iGEM24_OKstate   (2024-09-29)


Production of Chimeric Gp8 with S. aureus targeting peptide

This is a composite part we designed for our project. It combines a strong constitutive promoter, a rbs, a Dsba signaling sequence (for protein secretion across Sec system in E. coli), a S. aureus targeting peptide fused to a chimeric Gp8, and end with a terminator. This assembly for the S. aureus targeting Gp8 is the first of the three planned in our study.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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