Composite

Part:BBa_K5444007

Designed by: Ihsiu Huang   Group: iGEM24_OKstate   (2024-09-29)


Production of Chimeric Gp3 with S. aureus targeting peptide

This is a composite part we designed for our project. It combines a strong constitutive promoter, a rbs, a Dsba signaling sequence (for protein secretion across Sec system in E. coli), a S. aureus targeting peptide fused to a chimeric Gp3, and end with a terminator. This assembly for the S. aureus targeting Gp3 and is the second of the three planned in our study.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 153
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 153
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 153
    Illegal NgoMIV site found at 165
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None