Coding

Part:BBa_K5444004

Designed by: Ihsiu Huang   Group: iGEM24_OKstate   (2024-09-29)


Chimeric M13 Gp3

This is the coding sequencing for M13 Gp3. it encodes for the minor tail protein of the M13 phage. Historically Gp3 has been used as phage display support to identify peptides that binds to specific targets. We are using this part in our project to fuse with S. aureus targeting peptides at it's N-terminus. The resultant chimeric Gp3, when assembled with the rest of the phage proteins will be able to display the SA targeting peptides at the tail. We have included a 6XHis tag at the N-terminal ends for purification and detection (if so desired).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None