Signalling

Part:BBa_K5444000

Designed by: Ihsiu Huang   Group: iGEM24_OKstate   (2024-09-27)


DsbA signaling peptide (Sec secretion)

This ORF encodes for the DsbA signaling peptide. It allows any protein fused to the peptide to be translocated via the E. coli Sec secretion system. We are using it to tag our Gp3 and Gp8 phage proteins as part of the phagemid construct. The signaling peptide will allow the chimeric Gp3 and Gp8 to be secreted and later incorporated into the engineered M13 phage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyE. coli
signalling_moleculeDsbA signaling peptide