Plasmid_Backbone

Part:BBa_K5441007

Designed by: Lok Yin Wong   Group: iGEM24_PLKLFC   (2024-10-01)


pENTR1A


The pENTR1A vector is designed for easy cloning and manipulation of genes. It serves as a gateway entry vector, allowing researchers to insert a gene of interest into a compatible destination vector using a simple recombination process.

This is a mammalian plasmid, which contains a kanamycin resistance gene, and does not include any promoters. Therefore, it can be customised to include any promoter and reporter gene combination to cater for your project’s needs.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 746
    Illegal XbaI site found at 788
    Illegal PstI site found at 740
    Illegal PstI site found at 901
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 746
    Illegal NheI site found at 44
    Illegal PstI site found at 740
    Illegal PstI site found at 901
    Illegal NotI site found at 474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 746
    Illegal BamHI site found at 728
    Illegal XhoI site found at 779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 746
    Illegal XbaI site found at 788
    Illegal PstI site found at 740
    Illegal PstI site found at 901
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 746
    Illegal XbaI site found at 788
    Illegal PstI site found at 740
    Illegal PstI site found at 901
    Illegal NgoMIV site found at 636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
None