Regulatory

Part:BBa_K5441005:Design

Designed by: Lok Yin Wong   Group: iGEM24_PLKLFC   (2024-09-30)


PSMA_Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 630


Design Notes

Some bases in the PSMA promoter gene originated from the GenBank gene include illegal restriction sites defined by iGEM. They are changed to other bases. Transfection of a plasmid with the gene PSMA-GFP into cancerous cells successfully produced significant fluorescence which is observed under an inverted microscope capable of monitoring live cells.


Source

Homo sapiens prostate-specific membrane antigen (PSM) gene (AF007544.1)

References