Composite

Part:BBa_K5437022

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


Controlled expression of MVA, MEP, GPP, L-borneol Biosynthesis Pathway Genes in pBAD

Controlled expression of ACCT, HmgS, MK, PMK, MDD, IspD, IspE, IspF, IspG, IspH at a level lower than overexpression by the T7 promoter. These enzymes make up the MVA, MEP, GPP, and L-borneol synthesis pathways. Enzymes are promoted by the araBAD promoter and multiple ribosome binding sites (RBS).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2341
    Illegal NheI site found at 5734
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1807
    Illegal NgoMIV site found at 2032
    Illegal AgeI site found at 3727
    Illegal AgeI site found at 8011
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 661


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Categories
Parameters
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