Composite

Part:BBa_K5437019

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


Overexpression proteins for originally rate limiting steps of MVA, MEP, GPP, and L-borneol Biosynthe

Includes DXS, DXR, IDI, IspA, BbTPS3, AlP, and HmgR which were originally rate-limiting. Through overexpression from the T7 promoter, these enzymes contribute to the MVA, MEP, GPP, and L-borneol Synthesis Pathways by increasing production speed. Includes 2 T7 promoter and multiple RBS for the succesful transcription of overexpressed enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
    Illegal NotI site found at 5267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5412
    Illegal BglII site found at 9083
    Illegal BglII site found at 10861
    Illegal BglII site found at 11495
    Illegal BamHI site found at 4328
    Illegal XhoI site found at 11742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5412
    Illegal SpeI site found at 83
    Illegal PstI site found at 5234
    Illegal NgoMIV site found at 1913
    Illegal NgoMIV site found at 10959
    Illegal AgeI site found at 10164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6846
    Illegal BsaI.rc site found at 9759


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