Coding

Part:BBa_K5437013:Design

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


MVAPP decarboxylase converts mevalonate-5-diphosphate (MVAPP) into isopentenyl diphosphate (IPP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization via IDT was conducted to optimize sequence for E. coli production


Source

NCBI provided an unoptimized gene sequence that was also described in Metabolic Engineering Escherichia coli for the Production of Lycopene

References