Coding

Part:BBa_K5437001

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-22)


DXP Synthase (DXS) which turns G3P and pyruvate into 1-Deoxyxylulose-5-phosphate synthase (DXP))

1-Deoxyxylulose-5-phosphate synthase (Dxs) catalyzes the first and rate-limiting step in the methylerythritol phosphate (MEP) pathway for isoprenoid biosynthesis, and also contributes to pyridoxal 5-phosphate and thiamine biosynthesis. It uses thiamine diphosphate (ThDP) as a coenzyme to condense pyruvate and G3P into 1-deoxy-D-xylulose 5-phosphate. Overexpression of Dxs increases isoprenoid production but slows cell growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1825
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None