Coding

Part:BBa_K5436123

Designed by: Ayaka Sasaki, Shota Yamamoto, Yuto Toriyama, Hanna Watanabe, Ryojun Hayashizaki   Group: iGEM24_Waseda-Tokyo   (2024-09-27)


RBS+BIND-PETase(ID23)

BIND-System Module(BBa_K5436100)+PETase(ID23)(BBa_K5436016)

Inducing whole-cell biocatalysts “BIND-PETase”. It worked well as whole-cell biocatalysts and was a candidate for BIND-bearPETase.

For more detail, refer to BBa_K5436124 "BIND-bearPETase"

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 395
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None