Coding

Part:BBa_K5436122

Designed by: Ayaka Sasaki, Shota Yamamoto, Yuto Toriyama, Hanna Watanabe, Ryojun Hayashizaki   Group: iGEM24_Waseda-Tokyo   (2024-09-27)

Optimized RBS+BIND-PETase(ID22)


BIND-System Module(BBa_K5436100)+PETase(ID22)(BBa_K5436014)

Inducing whole-cell biocatalysts “BIND-PETase”. It worked well as whole-cell biocatalysts and was a candidate for BIND-bearPETase.

For more detail, please refer to BBa_K5436124 , Waseda-Tokyo 2024 Results and Engineering success.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 395
    Illegal NgoMIV site found at 628
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None