Coding

Part:BBa_K5436030

Designed by: Ryojun HAYASHIZAKI   Group: iGEM24_Waseda-Tokyo   (2024-09-27)

duraPETase


PETase variants designed by directed evolution with C-terminal 6xHistag. This is designed by Y. Cui et al.(2021) which work as whole-cell biocatalysts. Based on the mutations published by Y. Cui (2021), we converted them into amino acid sequences and them performed codon optimization for E. coli.

Please refer to BBa_K5436124.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None