Coding
Part:BBa_K5436030
Designed by: Ryojun HAYASHIZAKI Group: iGEM24_Waseda-Tokyo (2024-09-27)
duraPETase
PETase variants designed by directed evolution with C-terminal 6xHistag. This is designed by Y. Cui et al.(2021) which work as whole-cell biocatalysts.
Based on the mutations published by Y. Cui (2021), we converted them into amino acid sequences and them performed codon optimization for E. coli.
Please refer to BBa_K5436124.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |