Coding
CsgA

Part:BBa_K5436006

Designed by: Ryojun HAYASHIZAKI   Group: iGEM24_Waseda-Tokyo   (2024-09-25)

CsgA-taa


CsgA without stop codon, fusing enzymes into Curli Fiber. This is a version of BBa_K1583000 from iGEM15_TU_Delft, with the stop codon removed. This allows for the expression of the desired protein in a fused state following the Curli fiber formation factor CsgA.

Please refer to BBa_K5436124.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None