Composite

Part:BBa_K5427077:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-24)


Q-sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 837


Design Notes

The Q-sensor was designed with specific optimization for E. coli, including adjustments to the GC content and codon optimization. Optimization of the linkers was critical for the Q-sensor to ensure that the sensor itself promoted efficient binding and quenching with the target protein (in our case Keratin).

Source

BBa_K4755007 BBa_K4755006 BBa_K4755005 Kolossov, V. L., Spring, B. Q., Sokolowski, A., Conour, J. E., Clegg, R. M., Kenis, P. J., & Gaskins, H. R. (2008). Engineering redox-sensitive linkers for genetically encoded FRET-based biosensors. Experimental Biology and Medicine, 233(2), 238-248.