Composite

Part:BBa_K5427071:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)

pTac + RBS 1_12000 + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 88
    Illegal NheI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 899
    Illegal BsaI.rc site found at 72
    Illegal SapI.rc site found at 167


Design Notes

Not applicable.

Source

BBa_J23119

Ryu, M.-H., & Gomelsky, M. (2014). Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications. ACS Synthetic Biology, 3(11), 802–810. https://doi.org/10.1021/sb400182x

BBa_J428326