Primer

Part:BBa_K5427068:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_KerDZ_Pet22


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 47
    Illegal XbaI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 47
    Illegal NotI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 47
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 47
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 47
    Illegal XbaI site found at 5
    Illegal XbaI site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Forward primer for amplification of KerDZ insert in pET22b+.


Source

Not applicable.

References