Primer
Part:BBa_K5427068
Designed by: Brittany Green Group: iGEM24_UAlberta (2024-09-23)
FW_KerDZ_Pet22
Forward primer for amplification of KerDZ insert in pET22b+.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 47
Illegal XbaI site found at 5 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 47
Illegal NotI site found at 53 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 47
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 47
Illegal XbaI site found at 5 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 47
Illegal XbaI site found at 5
Illegal XbaI site found at 62 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |