Primer

Part:BBa_K5427066:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_Pet22_KerDZ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 36
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 37
    Illegal PstI site found at 51
    Illegal NotI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal PstI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Forward primer for linearization of pET22b+ with KerDZ.


Source

Not applicable.

References