Primer

Part:BBa_K5427063

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV 1234_ SS_pJump24 (I GEM_2024_11)

Reverse primer for Gibson Assembly of masP1, RBS1/2/3/4, and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
    Illegal NotI site found at 10
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None