Primer

Part:BBa_K5427062

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW4 _ SS_pJump24 (I GEM_2024_10)

Forward primer for Gibson Assembly of masP1 and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 51
    Illegal suffix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 51
    Illegal SpeI site found at 10
    Illegal PstI site found at 24
    Illegal NotI site found at 17
    Illegal NotI site found at 36
    Illegal NotI site found at 57
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 51
    Illegal XhoI site found at 45
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 51
    Illegal suffix found in sequence at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 51
    Illegal XbaI site found at 66
    Illegal SpeI site found at 10
    Illegal PstI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None