Primer
Part:BBa_K5427062
Designed by: Brittany Green Group: iGEM24_UAlberta (2024-09-23)
FW4 _ SS_pJump24 (I GEM_2024_10)
Forward primer for Gibson Assembly of masP1 and pJUMP24.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 51
Illegal suffix found in sequence at 9 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 51
Illegal SpeI site found at 10
Illegal PstI site found at 24
Illegal NotI site found at 17
Illegal NotI site found at 36
Illegal NotI site found at 57 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 51
Illegal XhoI site found at 45 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 51
Illegal suffix found in sequence at 10 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 51
Illegal XbaI site found at 66
Illegal SpeI site found at 10
Illegal PstI site found at 24 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |