Primer

Part:BBa_K5427061

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW3 _ SS_pJump24 (I GEM_2024_9)

Forward primer for Gibson Assembly of masP1 and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 49
    Illegal suffix found in sequence at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 8
    Illegal PstI site found at 22
    Illegal NotI site found at 15
    Illegal NotI site found at 34
    Illegal NotI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 49
    Illegal XhoI site found at 43
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 49
    Illegal suffix found in sequence at 8
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 49
    Illegal XbaI site found at 64
    Illegal SpeI site found at 8
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None