Primer

Part:BBa_K5427059

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW1 _ SS_pJump24 (I GEM_2024_7)

Forward primer for Gibson Assembly of masP1 and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 52
    Illegal suffix found in sequence at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 52
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 37
    Illegal NotI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 52
    Illegal XhoI site found at 46
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 52
    Illegal suffix found in sequence at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 52
    Illegal XbaI site found at 67
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None