Primer

Part:BBa_K5427058

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV_ pTac_RBS4 (I GEM_2024_6)

Reverse primer for Gibson Assembly of pTac and RBS4.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal SpeI site found at 90
    Illegal PstI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 90
    Illegal PstI site found at 76
    Illegal NotI site found at 41
    Illegal NotI site found at 62
    Illegal NotI site found at 81
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 49
    Illegal XhoI site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal SpeI site found at 90
    Illegal PstI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 49
    Illegal XbaI site found at 34
    Illegal SpeI site found at 90
    Illegal PstI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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