Primer

Part:BBa_K5427053

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_p JUMP24_pTac_RBS1234 (I GEM_2024_1)

Forward primer for Gibson Assembly of pTac, RBS1/2/3/4, and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 56
    Illegal PstI site found at 70
    Illegal NotI site found at 63
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 56
    Illegal PstI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None