Primer

Part:BBa_K5427050

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV_RBS3_sf GFP_p JUMP24 (I GEM_2024_29)

Reverse primer for Gibson Assembly of pTac RBS3 sfGFP in pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 41
    Illegal PstI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 41
    Illegal PstI site found at 27
    Illegal NotI site found at 32
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 41
    Illegal PstI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 41
    Illegal PstI site found at 27
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 11


[edit]
Categories
Parameters
None