Primer

Part:BBa_K5427048:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_pJump_24_RBS2_sfGFP (I GEM_2024_25)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
    Illegal NotI site found at 21
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43


Design Notes

Forward primer for Gibson Assembly of pTAC RBS1 sfGFP in pJUMP24.


Source

Not applicable.

References