Primer

Part:BBa_K5427046

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV_pJump_RBS1234_24_sfGFP (I GEM_2024_23)

Reverse primer for Gibson Assembly of RBS1/2/3/4, sfGFP, and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal XbaI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal NotI site found at 13
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal XbaI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal XbaI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None