Coding

Part:BBa_K5427004:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-22)


CelCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 125
    Illegal BglII site found at 261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To optimize the expression of CelCD in E. coli, we applied several strategies. Codon optimization was performed to enhance translational efficiency in the host. We ensured that the GC content was within the optimal range for E. coli expression (40%), and illegal restriction sites were removed to comply with Biobrick standards.


Source

Gao, D., Luan, Y., Wang, Q., Liang, Q., & Qi, Q. (2015). Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase. Microbial Cell Factories, 14, 1-8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712877/


References