Coding

Part:BBa_K5427001:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-22)

Keratin31


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To optimize the expression of Keratin31 in E. coli, we applied several strategies. Codon optimization was performed to enhance translational efficiency in the host. Repetitive sequences were manually removed to prevent issues with genetic instability. We ensured that the GC content was within the optimal range for E. coli expression (37%), and illegal restriction sites were removed to comply with Biobrick standards.


Source

Keratin 31_B0LKP0 · B0LKP0_SHEEP. UniProt. (n.d.). https://www.uniprot.org/uniprotkb/B0LKP0/entry

References