Composite

Part:BBa_K5426010

Designed by: Oliver Stearns   Group: iGEM24_Northeastern-Boston   (2024-10-01)


pET28a+ for Linalool Expression

A pET28a+ vector containing Linalool Synthase under a LacI/IPTG inducible expression system. For the production of Linalool from GPP. Was paired with pJBEI-6409 (Kealsing et al. 2013) which contains a pathway engineered to produce GPP in E.coli. Under a pMBI Origin of Replication. Contains a Kanamycin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1189
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1189
    Illegal NgoMIV site found at 1995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 482


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Categories
Parameters
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