Composite
Part:BBa_K5426010
Designed by: Oliver Stearns Group: iGEM24_Northeastern-Boston (2024-10-01)
pET28a+ for Linalool Expression
A pET28a+ vector containing Linalool Synthase under a LacI/IPTG inducible expression system. For the production of Linalool from GPP. Was paired with pJBEI-6409 (Kealsing et al. 2013) which contains a pathway engineered to produce GPP in E.coli. Under a pMBI Origin of Replication. Contains a Kanamycin resistance gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1189
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1189
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1189
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1189
Illegal NgoMIV site found at 1995 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 482
[edit]
Categories
Parameters
None |