Composite

Part:BBa_K5426009

Designed by: Oliver Stearns   Group: iGEM24_Northeastern-Boston   (2024-10-01)


pET28a+ for Expression in E.coli

A pET28a+ vector containing a pMBI origin of replication along with a Lac/IPTG induction system specifically engineered for the production of proteins into E.coli. Comes with a Kanamycin resistance marker for counter selection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 182
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None