Part:BBa_K5422004
T4 endolysin
Usage:
In the Bac'Attack Aix-Marseille University team's project, T4 endolysin is employed to lyse bacteria and release the toxin of interest, Cyt2Aa1 (see BBa_K5422003). T4 endolysin is an enzyme derived from bacteriophage T4, specifically designed to degrade the bacterial cell wall by targeting peptidoglycans. In our system, the enzyme facilitates the controlled release of Cyt2Aa1 into the environment, maximizing the effectiveness of the insecticidal agent. To enhance purification and detection, a His-tag was added to the C-terminal region of the endolysin.
Biology:
T4 endolysin is a lytic enzyme produced by bacteriophage T4 to facilitate the release of newly formed viral particles by breaking down the bacterial cell wall. The enzyme specifically targets peptidoglycan, a major structural component of the bacterial cell envelope, which provides rigidity and protection. By cleaving the glycosidic bonds in peptidoglycan, T4 endolysin rapidly breaks down the bacterial wall, leading to cell lysis. This enzyme is highly efficient at inducing bacterial lysis and is often used in synthetic biology and biotechnology applications to control bacterial populations or release intracellular products.
Structurally, T4 endolysin typically consists of two domains: a catalytic domain, which actively degrades the peptidoglycan layer, and a cell wall binding domain (CBD), which ensures that the enzyme targets the bacterial cell wall specifically. This precise targeting mechanism makes endolysins effective tools for the controlled lysis of bacteria without harming surrounding non-target cells, providing a clean and specific mechanism for bacterial cell destruction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 271
Illegal AgeI site found at 341 - 1000COMPATIBLE WITH RFC[1000]
| None |