Coding

Part:BBa_K542010:Design

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-26)

Enhanced Lumazine Synthase (ELS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The compartment formed by this polypeptide has a larger cavity than the Lumazine Synthase microcompartment submitted by the Lethbridge 2009 team (BBa_K249002). A larger interior allows for the option of localizing larger and/or more proteins into the cavity of the compartment.


Source

The part was synthesized by [http://www.biobasic.com/ Bio Basic Inc.] and moved into pSB1C3 by the Lethbridge 2011 team.

References

Wörsdörfer, B., Woycechowsky, K.J., and Hilvert, D., (2011). Directed Evolution of a Protein Container. Science. 331: 589-592.