Intermediate

Part:BBa_K542007

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-24)

Unregulated TetR with pTet Regulated Arg-tagged ECFP

Made by assembling BBa_P0440 with BBa_K331033.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.

Intermediate part to assemble the "Co-localization Testing Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None