Plasmid

Part:BBa_K541925:Design

Designed by: Mustafa Elitok   Group: iGEM11_Fatih_Turkey   (2011-09-21)


Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5543
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5543
    Illegal NheI site found at 694
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5549
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5543
    Illegal BglII site found at 3754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5543
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5543
    Illegal XbaI site found at 5558
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3578


Design Notes

Optimized for B.subtilis


Source

Horseshoe crab

References