Plasmid_Backbone

Part:BBa_K5419013

Designed by: ALEXIA XINYUE ZHAO   Group: iGEM24_Foshan-GreatBay   (2024-08-17)


X-3-XI-2-gRNA-Backbone



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal NheI site found at 1165
    Illegal NheI site found at 1607
    Illegal NheI site found at 3850
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal BamHI site found at 1428
    Illegal XhoI site found at 1862
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1582
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 4131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3027
    Illegal SapI site found at 1944

<!DOCTYPE html> BBa_K5419013 (X-3-XI-2-gRNA-Backbone)

BBa_K5419013 (X-3-XI-2-gRNA-Backbone)

Profile

Name X-3-XI-2-gRNA-Backbone
Base Pairs 6379 bp
Origin Artificial gene synthesis
Properties It encodes gRNA that can combine with Cas9 protein and guide Cas9 protein to the X-3 and XI-2 gene sites, and then Cas9 protein cuts a length of DNA.
Figure 1: Plasmid map of X-3-XI-2-gRNA-Backbone
Figure 1 Plasmid map of X-3-XI-2-gRNA-Backbone

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Categories
Parameters
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