Plasmid_Backbone

Part:BBa_K5419012

Designed by: ALEXIA XINYUE ZHAO   Group: iGEM24_Foshan-GreatBay   (2024-08-17)


XI-2-gRNA-Backbone


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1503
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 3760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal NheI site found at 1165
    Illegal NheI site found at 3479
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 3760
    Illegal NotI site found at 1484
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal BamHI site found at 1428
    Illegal XhoI site found at 1491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1503
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 3760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 430
    Illegal EcoRI site found at 1458
    Illegal XbaI site found at 1140
    Illegal XbaI site found at 1417
    Illegal XbaI site found at 1503
    Illegal SpeI site found at 1026
    Illegal PstI site found at 340
    Illegal PstI site found at 855
    Illegal PstI site found at 1463
    Illegal PstI site found at 3760
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2656
    Illegal SapI site found at 1573


BBa_K5419011 (XI-2-gRNA-Backbone)

BBa_K5419011 (XI-2-gRNA-Backbone)

Profile

Name XI-2-gRNA-Backbone
Base Pairs 6008 bp
Origin Artificial gene synthesis
Properties It encodes gRNA that can combine with Cas9 protein and guide Cas9 protein to the XI-2 site, and then Cas9 protein cuts a length of DNA.
Figure 1: Plasmid map of XI-2-gRNA-Backbone
Figure 1 Plasmid map of XI-2-gRNA-Backbone

[edit]
Categories
Parameters
None