Plasmid_Backbone

Part:BBa_K5419010

Designed by: ALEXIA XINYUE ZHAO   Group: iGEM24_Foshan-GreatBay   (2024-08-17)

pCas9-G418


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4260
    Illegal PstI site found at 681
    Illegal PstI site found at 2649
    Illegal PstI site found at 2883
    Illegal PstI site found at 4095
    Illegal PstI site found at 4399
    Illegal PstI site found at 5245
    Illegal PstI site found at 7757
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4260
    Illegal PstI site found at 681
    Illegal PstI site found at 2649
    Illegal PstI site found at 2883
    Illegal PstI site found at 4095
    Illegal PstI site found at 4399
    Illegal PstI site found at 5245
    Illegal PstI site found at 7757
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4260
    Illegal BglII site found at 688
    Illegal BglII site found at 1625
    Illegal BglII site found at 5035
    Illegal BamHI site found at 2443
    Illegal XhoI site found at 3981
    Illegal XhoI site found at 4586
    Illegal XhoI site found at 4601
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4260
    Illegal PstI site found at 681
    Illegal PstI site found at 2649
    Illegal PstI site found at 2883
    Illegal PstI site found at 4095
    Illegal PstI site found at 4399
    Illegal PstI site found at 5245
    Illegal PstI site found at 7757
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4260
    Illegal PstI site found at 681
    Illegal PstI site found at 2649
    Illegal PstI site found at 2883
    Illegal PstI site found at 4095
    Illegal PstI site found at 4399
    Illegal PstI site found at 5245
    Illegal PstI site found at 7757
    Illegal NgoMIV site found at 3177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3323
    Illegal BsaI site found at 4177
    Illegal BsaI site found at 9586
    Illegal BsaI.rc site found at 168
    Illegal BsaI.rc site found at 1848
    Illegal BsaI.rc site found at 7067
    Illegal SapI site found at 7219
    Illegal SapI.rc site found at 8564
    Illegal SapI.rc site found at 10668


BBa_K5419010 (pCas9-G418-Backbone)

BBa_K5419010 (pCas9-G418-Backbone)

Profile

Name pCas9-G418-Backbone
Base Pairs 10949 bp
Origin Artificial gene synthesis
Properties It is a synthetic gene that codes for Cas9. It encodes the Cas9 protein that can combine with gRNA, which then leads the Cas9 protein to a specific site of genomes of the yeast and cuts out the gene.
Figure 1: Plasmid map of pCas9-G418-Backbone
Figure 1 Plasmid map of pCas9-G418-Backbone

[edit]
Categories
Parameters
None