Composite

Part:BBa_K5416062:Design

Designed by: Jixiao Wu   Group: iGEM24_Imperial-College   (2024-09-30)


pT7LacO + P22 + HRT2-SP + T7term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 636
    Illegal AgeI site found at 1037
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon and RBSs are optimized for expression of this specific part in E. coli


Source

Sourced from part BBa_K2406020; BBa_K5416061; BBa_K5416001; BBa_K731721

References