Signalling
HIF-1β
Part:BBa_K5415000:Design
Designed by: Agnese Gabriela Celma Group: iGEM24_Latvia-Riga (2024-09-29)
HIF-1β, a subunit linked to HIF-1α, regulates hypoxia response.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 572
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ARNT sequence must be designed to ensure high affinity and specificity for hypoxia response elements (HREs) as well as a high dimerization compatibility, both of which are critical for regulating gene expression under low oxygen conditions, as these interactions are pivotal for activating transcription of target genes.
Source
The protein sequence was acquired from UniProt (ID P27540) and converted to a DNA sequence via VectorBuilder and optimised for S.cerevisiae. Gibson and in vivo cloning methods were employed.