Translational_Unit

Part:BBa_K5410028

Designed by: Zixuan Li   Group: iGEM24_GeorgiaState-SWJTU   (2024-10-02)


phaBCA for E. coli Poly-3-hydroxybutyrate production

A modified E. coli strain with an operon of three genes (organized as phaCAB, from Ralstonia eutropha, also called as Cupriavidus necator N-1) can be used for the production of Poly-3-hydroxybutyrate (PHB). The three genes of the phaCAB operon are phaC, which encodes the polyhydroxyalkanoate (PHA) synthase, phaA, which encodes a 3-ketothiolase, and phaB, which encodes an acetoacetyl coenzyme A (acetoacetyl-CoA) reductase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
    Illegal NotI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1328
    Illegal XhoI site found at 133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1510
    Illegal PstI site found at 2083
    Illegal NgoMIV site found at 94
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 472
    Illegal NgoMIV site found at 939
    Illegal NgoMIV site found at 1054
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 1879
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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