Plasmid
Part:BBa_K5407008
Designed by: Fangyuan Duan Group: iGEM24_YiYe-Wuhan (2024-09-16)
pMMC plasmid
Usage and Biology
pMMC plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 8307 bp,and the selectable markers of the plasmid is Ampicillin. Scientists often use the plasmid tool to constitutive expression gene from a Pc promoter. In our study, we cloned the nuclear translocation of TEAD4, namely NLS (nuclear location sequence) into the basic part pMMC plasmid (figure 1). The NLS method means destroyed the protein nuclear location ability by deleted the 105aa to 109aa of TEAD4, therefore, TEAD4 unable to get into the nuclear and function as transcription factor.
Figure 1 pMMC map
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 469
Illegal PstI site found at 491 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 469
Illegal PstI site found at 491 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 469
Illegal BamHI site found at 479 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 469
Illegal PstI site found at 491 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 469
Illegal PstI site found at 491
Illegal NgoMIV site found at 289
Illegal AgeI site found at 3120
Illegal AgeI site found at 3521 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 671
Illegal BsaI site found at 1738
Illegal SapI site found at 7730
[edit]
Categories
Parameters
None |