Plasmid

Part:BBa_K5407008

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-Wuhan   (2024-09-16)


pMMC plasmid

Usage and Biology

pMMC plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 8307 bp,and the selectable markers of the plasmid is Ampicillin. Scientists often use the plasmid tool to constitutive expression gene from a Pc promoter. In our study, we cloned the nuclear translocation of TEAD4, namely NLS (nuclear location sequence) into the basic part pMMC plasmid (figure 1). The NLS method means destroyed the protein nuclear location ability by deleted the 105aa to 109aa of TEAD4, therefore, TEAD4 unable to get into the nuclear and function as transcription factor.


pmmc.png 

                              Figure 1 pMMC map



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 469
    Illegal PstI site found at 491
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 469
    Illegal PstI site found at 491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 469
    Illegal BamHI site found at 479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 469
    Illegal PstI site found at 491
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 469
    Illegal PstI site found at 491
    Illegal NgoMIV site found at 289
    Illegal AgeI site found at 3120
    Illegal AgeI site found at 3521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 671
    Illegal BsaI site found at 1738
    Illegal SapI site found at 7730


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